Agrégats de nanoparticules allongées dans les cellules cancéreuses pour la destruction mécanique avec un champ magnétique rotatif à basse fréquence
Yajing Shen, Congyu Wu, Taro QP Uyeda, Gustavo R Plaza, Bin Liu, Yu Han, Maciej S Lesniak, Yu Cheng
Résumé
Magnetic nanoparticles (MNPs) functionalized with targeting moieties can recognize specific cell components and induce mechanical actuation under magnetic field. Their size is adequate for reaching tumors and targeting cancer cells. However, due to the nanometric size, the force generated by MNPs is smaller than the force required for largely disrupting key components of cells. Here, we show the magnetic assembly process of the nanoparticles inside the cells, to form elongated aggregates with the size required to produce elevated mechanical forces. We synthesized iron oxide nanoparticles doped with zinc, to obtain high magnetization, and functionalized with the epidermal growth factor (EGF) peptide for targeting cancer cells. Under a low frequency rotating magnetic field at 15 Hz and 40 mT, the internalized EGF-MNPs formed elongated aggregates and generated hundreds of pN to dramatically damage the plasma and lysosomal membranes. The physical disruption, including leakage of lysosomal hydrolases into the cytosol, led to programmed cell death and necrosis. Our work provides a novel strategy of designing magnetic nanomedicines for mechanical destruction of cancer cells.
Keywords: Brain cancer cells; Functionalized magnetic nanoparticles; Lysosome damage; Magneto-mechanical actuation; Plasma membrane damage.
Related products
Abbreviation: mPEG-NH2
Name: Methoxypoly(ethylene glycol) amine
US Tel: 1-844-782-5734
US Tel: 1-844-QUAL-PEG
CHN Tel: 400-918-9898
Email: sales@sinopeg.com
